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cd5 (pecy5  (Immunostep)

 
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    Immunostep cd5 (pecy5
    Cd5 (Pecy5, supplied by Immunostep, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd5+(pecy5/monoclonal+anti+mouse+antibody+cd3+pecy5/pmc05217033-225-66-81
    Average 90 stars, based on 1 article reviews
    cd5 (pecy5 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: EphrinA4 plays a critical role in α4 and αL mediated survival of human CLL cells during extravasation
    Article Snippet: Acquisition was performed at low speed for 1 min. Absolute cell counts were determined according to the following formula: (Number of B-cell events / Number of bead events) × number of beads added For immunofluorescent staining cell suspensions were incubated in cold PBS [0.1% bovine serum albumin (BSA)] (2×10 5 cells/50 μL) with saturating amounts of antibodies to human antigens including: anti-CD19 (FITC, APC or PE), -CD5 (PECy5); FITC or PE-Cy5 anti-CD11a (αL;), -CD29 (β1), -CD18 (β2) or -CD49d (α4)(all from ImmunoStep, Spain); PE conjugated anti ZAP-70 or APC-CD38 (BD).

    Incubation:

    Article Title: EphrinA4 plays a critical role in α4 and αL mediated survival of human CLL cells during extravasation
    Article Snippet: Acquisition was performed at low speed for 1 min. Absolute cell counts were determined according to the following formula: (Number of B-cell events / Number of bead events) × number of beads added For immunofluorescent staining cell suspensions were incubated in cold PBS [0.1% bovine serum albumin (BSA)] (2×10 5 cells/50 μL) with saturating amounts of antibodies to human antigens including: anti-CD19 (FITC, APC or PE), -CD5 (PECy5); FITC or PE-Cy5 anti-CD11a (αL;), -CD29 (β1), -CD18 (β2) or -CD49d (α4)(all from ImmunoStep, Spain); PE conjugated anti ZAP-70 or APC-CD38 (BD).



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    Analysis of splenic B cells. A. Representative density plot of splenocytes from a WT-SLD mouse that shows the selection of B cells according to <t>CD5</t> and CD19 expression, which allowed the determination of three populations: CD19 - CD5 + (region 1), CD19 - CD5 - (region 2) and CD19 + CD5 +/- (region 3, B cells). Representative histograms of the expression of B. CD3, C. CD11b and D. CD11c on splenocytes from a WT-SLD mouse in the regions indicated in A .
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    Analysis of splenic B cells. A. Representative density plot of splenocytes from a WT-SLD mouse that shows the selection of B cells according to <t>CD5</t> and CD19 expression, which allowed the determination of three populations: CD19 - CD5 + (region 1), CD19 - CD5 - (region 2) and CD19 + CD5 +/- (region 3, B cells). Representative histograms of the expression of B. CD3, C. CD11b and D. CD11c on splenocytes from a WT-SLD mouse in the regions indicated in A .
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    cd5-pecy5 (catalog no. 53-7.3)  (Becton Dickinson)
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    Analysis of splenic B cells. A. Representative density plot of splenocytes from a WT-SLD mouse that shows the selection of B cells according to <t>CD5</t> and CD19 expression, which allowed the determination of three populations: CD19 - CD5 + (region 1), CD19 - CD5 - (region 2) and CD19 + CD5 +/- (region 3, B cells). Representative histograms of the expression of B. CD3, C. CD11b and D. CD11c on splenocytes from a WT-SLD mouse in the regions indicated in A .
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    Image Search Results


    Analysis of splenic B cells. A. Representative density plot of splenocytes from a WT-SLD mouse that shows the selection of B cells according to CD5 and CD19 expression, which allowed the determination of three populations: CD19 - CD5 + (region 1), CD19 - CD5 - (region 2) and CD19 + CD5 +/- (region 3, B cells). Representative histograms of the expression of B. CD3, C. CD11b and D. CD11c on splenocytes from a WT-SLD mouse in the regions indicated in A .

    Journal: Data in Brief

    Article Title: Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis

    doi: 10.1016/j.dib.2016.02.048

    Figure Lengend Snippet: Analysis of splenic B cells. A. Representative density plot of splenocytes from a WT-SLD mouse that shows the selection of B cells according to CD5 and CD19 expression, which allowed the determination of three populations: CD19 - CD5 + (region 1), CD19 - CD5 - (region 2) and CD19 + CD5 +/- (region 3, B cells). Representative histograms of the expression of B. CD3, C. CD11b and D. CD11c on splenocytes from a WT-SLD mouse in the regions indicated in A .

    Article Snippet: The following monoclonal antibodies were purchased from Becton Dickinson Bioscience (BD Bioscience, CA): CD3e-FITC (145-2C11), CD4-FITC (RM4-5), CD5-PECy5 (53-7.3), CD8a-PE (53-6.7), CD11b-APC (M1/70), CD11c-APC (HL3), CD19-PECy7 (1D3), CD21-FITC (7G6), CD23-PE (B3B4), CD24-APC (M1/69), CD40-APC (3/23), CD45-APCCy7 (30-F11), CD49b/Pan-NK-FITC (DX5), CD80-APC (16-10A1), CD86-APC (GL1), CD95-PE (15A7), IgM-biotin (II/41), IgD-V450 (11-26c.2a), Ter-119-PE (TER-119), B220-Pacifc Blue (RA3-6B2), and the respective isotype controls.

    Techniques: Selection, Expressing

    B cells from para-aortic and mediastinal lymph nodes in mice with atherosclerosis. A. Representative analysis of B cell subpopulations from lymph nodes divided in CD5 + , T1-like and FO, from WT-SLD mouse by flow cytometry. Mean fluorescence intensity (MFI) of CD19 on B cells from B. para-aortic and C. mediastinal lymph nodes. Frequency of B cell subpopulations in D. para-aortic and E. mediastinal lymph nodes. Mean and data from 3-4 mice per group are shown. Analysis in lymph nodes was performed as in spleens. Two-way ANOVA with Šídák post-test were performed.

    Journal: Data in Brief

    Article Title: Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis

    doi: 10.1016/j.dib.2016.02.048

    Figure Lengend Snippet: B cells from para-aortic and mediastinal lymph nodes in mice with atherosclerosis. A. Representative analysis of B cell subpopulations from lymph nodes divided in CD5 + , T1-like and FO, from WT-SLD mouse by flow cytometry. Mean fluorescence intensity (MFI) of CD19 on B cells from B. para-aortic and C. mediastinal lymph nodes. Frequency of B cell subpopulations in D. para-aortic and E. mediastinal lymph nodes. Mean and data from 3-4 mice per group are shown. Analysis in lymph nodes was performed as in spleens. Two-way ANOVA with Šídák post-test were performed.

    Article Snippet: The following monoclonal antibodies were purchased from Becton Dickinson Bioscience (BD Bioscience, CA): CD3e-FITC (145-2C11), CD4-FITC (RM4-5), CD5-PECy5 (53-7.3), CD8a-PE (53-6.7), CD11b-APC (M1/70), CD11c-APC (HL3), CD19-PECy7 (1D3), CD21-FITC (7G6), CD23-PE (B3B4), CD24-APC (M1/69), CD40-APC (3/23), CD45-APCCy7 (30-F11), CD49b/Pan-NK-FITC (DX5), CD80-APC (16-10A1), CD86-APC (GL1), CD95-PE (15A7), IgM-biotin (II/41), IgD-V450 (11-26c.2a), Ter-119-PE (TER-119), B220-Pacifc Blue (RA3-6B2), and the respective isotype controls.

    Techniques: Flow Cytometry, Fluorescence

    Analysis of splenic B cell subpopulations. A. Representative analysis of splenic B cell subpopulations from a WT-SLD mouse. According to the expression of CD5, CD19, CD21, CD23 and CD24, lymphocytes were divided into CD5 + , MZ, T1-like, T2 and FO subsets. MFI of B. CD19 and C. CD21 on B cell subpopulations. MFI from 11-12 mice per group are shown from three independent experiments. D. Representative density plot of B220 expression on CD5 + B cells of a WT-SLD mouse. E. Frequency of B1a and no-B1a subpopulations in splenic B cells. F. Representative density plot of B220 expression on T1-like cells of a WT-SLD mouse. G. Frequency of B1b and T1 subpopulations in splenic B cells. Mean and data from 7-8 mice per group are shown. Two-way ANOVA with Šídák post-test were performed.

    Journal: Data in Brief

    Article Title: Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis

    doi: 10.1016/j.dib.2016.02.048

    Figure Lengend Snippet: Analysis of splenic B cell subpopulations. A. Representative analysis of splenic B cell subpopulations from a WT-SLD mouse. According to the expression of CD5, CD19, CD21, CD23 and CD24, lymphocytes were divided into CD5 + , MZ, T1-like, T2 and FO subsets. MFI of B. CD19 and C. CD21 on B cell subpopulations. MFI from 11-12 mice per group are shown from three independent experiments. D. Representative density plot of B220 expression on CD5 + B cells of a WT-SLD mouse. E. Frequency of B1a and no-B1a subpopulations in splenic B cells. F. Representative density plot of B220 expression on T1-like cells of a WT-SLD mouse. G. Frequency of B1b and T1 subpopulations in splenic B cells. Mean and data from 7-8 mice per group are shown. Two-way ANOVA with Šídák post-test were performed.

    Article Snippet: The following monoclonal antibodies were purchased from Becton Dickinson Bioscience (BD Bioscience, CA): CD3e-FITC (145-2C11), CD4-FITC (RM4-5), CD5-PECy5 (53-7.3), CD8a-PE (53-6.7), CD11b-APC (M1/70), CD11c-APC (HL3), CD19-PECy7 (1D3), CD21-FITC (7G6), CD23-PE (B3B4), CD24-APC (M1/69), CD40-APC (3/23), CD45-APCCy7 (30-F11), CD49b/Pan-NK-FITC (DX5), CD80-APC (16-10A1), CD86-APC (GL1), CD95-PE (15A7), IgM-biotin (II/41), IgD-V450 (11-26c.2a), Ter-119-PE (TER-119), B220-Pacifc Blue (RA3-6B2), and the respective isotype controls.

    Techniques: Expressing

    Analysis of B cells of aortic tissue. A. Representative density plot of CD21 and CD23 expression on splenic cells from a WT-SLD mouse with or without enzymatic cocktail treatment. B. Representative selection of aortic lymphocytes from a WT-SLD mouse according to the SSC-A and FSC-A parameters, with a typical CD45 expression of aortic lymphocyte cells (Red hystogram) and their analysis based on CD5 and CD19 expression. C. Representative density plots of the analysis of aortic B cell subpopulations in a WT-SLD mouse. According to the expression of CD19, B220, CD5, CD24, IgD and IgM, lymphocytes were divided into B1a, CD5 + non-B1a, B1b, FO, MZ and T1 subsets.

    Journal: Data in Brief

    Article Title: Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis

    doi: 10.1016/j.dib.2016.02.048

    Figure Lengend Snippet: Analysis of B cells of aortic tissue. A. Representative density plot of CD21 and CD23 expression on splenic cells from a WT-SLD mouse with or without enzymatic cocktail treatment. B. Representative selection of aortic lymphocytes from a WT-SLD mouse according to the SSC-A and FSC-A parameters, with a typical CD45 expression of aortic lymphocyte cells (Red hystogram) and their analysis based on CD5 and CD19 expression. C. Representative density plots of the analysis of aortic B cell subpopulations in a WT-SLD mouse. According to the expression of CD19, B220, CD5, CD24, IgD and IgM, lymphocytes were divided into B1a, CD5 + non-B1a, B1b, FO, MZ and T1 subsets.

    Article Snippet: The following monoclonal antibodies were purchased from Becton Dickinson Bioscience (BD Bioscience, CA): CD3e-FITC (145-2C11), CD4-FITC (RM4-5), CD5-PECy5 (53-7.3), CD8a-PE (53-6.7), CD11b-APC (M1/70), CD11c-APC (HL3), CD19-PECy7 (1D3), CD21-FITC (7G6), CD23-PE (B3B4), CD24-APC (M1/69), CD40-APC (3/23), CD45-APCCy7 (30-F11), CD49b/Pan-NK-FITC (DX5), CD80-APC (16-10A1), CD86-APC (GL1), CD95-PE (15A7), IgM-biotin (II/41), IgD-V450 (11-26c.2a), Ter-119-PE (TER-119), B220-Pacifc Blue (RA3-6B2), and the respective isotype controls.

    Techniques: Expressing, Selection

    Lipid accumulation in splenocytes exposed to lipemic serum. Representative histograms of A. T cells (CD19 - CD5 + ), B. B cells (CD19 + ) and C. CD11b + myeloid cells (CD19 - CD5 - CD11b + ) at 0, 5 and 12 h of culture of splenocytes from apoE -/- mice of 8 weeks old with serum from apoE -/- HFD or WT-SLD mice. The dotted line indicates the MFI of Nile Red at the beginning of the culture (0 h).

    Journal: Data in Brief

    Article Title: Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis

    doi: 10.1016/j.dib.2016.02.048

    Figure Lengend Snippet: Lipid accumulation in splenocytes exposed to lipemic serum. Representative histograms of A. T cells (CD19 - CD5 + ), B. B cells (CD19 + ) and C. CD11b + myeloid cells (CD19 - CD5 - CD11b + ) at 0, 5 and 12 h of culture of splenocytes from apoE -/- mice of 8 weeks old with serum from apoE -/- HFD or WT-SLD mice. The dotted line indicates the MFI of Nile Red at the beginning of the culture (0 h).

    Article Snippet: The following monoclonal antibodies were purchased from Becton Dickinson Bioscience (BD Bioscience, CA): CD3e-FITC (145-2C11), CD4-FITC (RM4-5), CD5-PECy5 (53-7.3), CD8a-PE (53-6.7), CD11b-APC (M1/70), CD11c-APC (HL3), CD19-PECy7 (1D3), CD21-FITC (7G6), CD23-PE (B3B4), CD24-APC (M1/69), CD40-APC (3/23), CD45-APCCy7 (30-F11), CD49b/Pan-NK-FITC (DX5), CD80-APC (16-10A1), CD86-APC (GL1), CD95-PE (15A7), IgM-biotin (II/41), IgD-V450 (11-26c.2a), Ter-119-PE (TER-119), B220-Pacifc Blue (RA3-6B2), and the respective isotype controls.

    Techniques: